Recombinant Human LRG1, His tagged

• Cat.No.: LRG1-141H
• Product Overview: Recombinant Human LRG1 (AAH34389.1) (Met1-Gln347), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 1-347 a.a.
• Predicted N Terminal: Val 36
• Form: Lyophilized from sterile PBS, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human LRG1 comprises 323 amino acids and has a predicted molecular mass of 35.8 kDa. The apparent molecular mass of the protein is approximately 44-48 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: LRG1 leucine-rich alpha-2-glycoprotein 1 [ Homo sapiens ]
• Official Symbol: LRG1
• Gene ID: 116844
• mRNA Refseq: NM_052972
• Protein Refseq: NP_443204
• MIM: 611289
• UniProt ID: P02750
• Chromosome Location: 19

Citation

The fetal mouse metatarsal bone explant as a model of angiogenesis.

Journal: Nature protocols    PubMed ID: 26334866    Data: 2016/4/5

Authors: Weihua Song, Chee Wai Fhu, Xiaomeng Wang

Article Snippet:DPBS with CaCl2 and MgCl2 maintains the structural and physiological integrity of the explant.. FBS (EU-approved; GE Healthcare, cat. no. A15-101) MEMα (1×), GlutaMAX (Minimum Essential Medium; Gibco, cat. no. 32561-037) DMEM, GlutaMAX (Gibco, cat. no. 10567-014) EGM-2 BulletKit (Lonza, cat. no. CC-3162) EBM-2 basal medium (Lonza, cat. no. CC-3162) Penicillin-streptomycin solution (100×; 10,000-U ml?1 penicillin, 10,000-μg ml?1 streptomycin; Thermo Scientific HyClone, cat. no. SV30010) ? ? ? ? ? ? ? ? 20 15 N at u re A m er ic a, In c. A ll ri g h ts r es er ve d . 1464 | VOL.10 NO.10 | 2015 | nature protocols HBSS 1× (Hank’s balanced salt solution + CaCl2 + MgCl2; Gibco, cat. no. 24020-117) EmbryoMax ultrapure water with 0.1% (wt/vol) gelatin (Millipore, cat. no. ES-006-B) Collagen type I, rat tail, 100 mg, 3 mg ml?1 (Corning, cat. no. 354236) Fibronectin from bovine plasma (Sigma-Aldrich, cat. no. F1141-5MG) ALK1 inhibitors (LDN 193189; Axon Medchem BV, cat. no. 1509-B5) Recombinant LRG1 (HLRG1-His; produced in house, also available from Creative BioMart, cat. no. LRG1-2295H) Paraformaldehyde (PFA; Sigma-Aldrich, cat. no. 158127-500G) ! cautIon PFA is harmful if inhaled or swallowed.. It may sensitize skin upon contact, and it is carcinogenic.