Recombinant Human LRRTM4 Protein, Gln31-Lys424, C-6×His tagged

• Cat.No.: LRRTM4-07H
• Product Overview: Recombinant Human LRRTM4 Protein, Gln31-Lys424, with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gln31-Lys424
• Description: Predicted to enable heparan sulfate proteoglycan binding activity. Predicted to be involved in regulation of synapse assembly. Predicted to act upstream of or within AMPA glutamate receptor clustering; positive regulation of synapse assembly; and regulation of presynaptic membrane organization. Predicted to be located in postsynaptic membrane. Predicted to be part of AMPA glutamate receptor complex. Predicted to be active in extracellular matrix; extracellular space; and glutamatergic synapse. Predicted to be integral component of postsynaptic density membrane.
• Bio-activity: Measured by its binding ability in a functional ELISA. When rhLRRTM4 is coated at 5 μg/mL (100 μL/well), rhNeurexin 1 beta /Fc Chimera binds with an apparent KD <15 nM.
• Molecular Mass: Predicted Molecular Mass: 46.0 kDa
  SDS-PAGE: 60-70 kDa, reducing conditions
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• N-terminal Sequence Analysis: No results obtained: Gln31 predicted
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 300 μg/mL in PBS.
• References: 1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Linhoff, M.W. et al. (2009) Neuron 61:734.
  3. Majercak, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17967.

Gene Information

• Gene Name: LRRTM4 leucine rich repeat transmembrane neuronal 4 [ Homo sapiens (human) ]
• Official Symbol: LRRTM4
• Synonyms: LRRTM4; leucine rich repeat transmembrane neuronal 4; leucine-rich repeat transmembrane neuronal protein 4; FLJ12568; leucine-rich repeat transmembrane neuronal 4 protein; MGC120633; MGC120636
• Gene ID: 80059
• mRNA Refseq: NM_024993
• Protein Refseq: NP_079269
• MIM: 610870
• UniProt ID: Q86VH4