Recombinant Human MADCAM1 protein, His-tagged

• Cat.No.: MADCAM1-2538H
• Product Overview: Recombinant Human MADCAM1 protein(NP_570116.2)(19-317aa), fused to His-tag at C-terminus, was expressed in Yeast.

Specification

• Description: The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined.
• Source: Yeast
• Species: Human
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 33.4 kDa
• Protein length: 19-317aa
• AA Sequence: QSLQVKPLQVEPPEPVVAVALGASR QLTCRLACADRGASVQWRGLDTSLG AVQSDTGRSVLTVRNASLSAAGTRV CVGSCGGRTFQHTVQLLVYAFPDQL TVSPAALVPGDPEVACTAHKVTPVD PNALSFSLLVGGQELEGAQALGPEV QEEEEEPQGDEDVLFRVTERWRLPP LGTPVPPALYCQATMRLPGLELSHR QAIPVLHSPTSPEPPDTTSPESPDT TSPESPDTTSQEPPDTTSPEPPDKT SPEPAPQQGSTHTPRSPGSTRTRRP EISQAGPTQGEVIPTGSSKPAGDQ
• Purity: Greater than 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens (human) ]
• Official Symbol: MADCAM1
• Synonyms: Addressin mucosal; hMAdCAM 1 ; hMAdCAM-1; MACAM1; MADCA_HUMAN; MAdCAM 1; MAdCAM-1; Madcam1
• Gene ID: 8174
• mRNA Refseq: NM_130760.3
• Protein Refseq: NP_570116.2
• MIM: 102670
• UniProt ID: Q13477