Recombinant Human MAGEA4 protein, GST-tagged

• Cat.No.: MAGEA4-6573H
• Product Overview: Recombinant Human MAGEA4 protein(1-317 aa), fused with N-terminal GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1-317 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 8.0). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 100 mM reduced glutathione (GSH).
• AASequence: MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPTSNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: MAGEA4 melanoma antigen family A, 4 [ Homo sapiens ]
• Official Symbol: MAGEA4
• Synonyms: MAGEA4; melanoma antigen family A, 4; MAGE4; melanoma-associated antigen 4; cancer/testis antigen family 1; member 4; CT1.4; MAGE 41; MAGE X2; MAGE4A; MAGE4B; melanoma associated antigen 4; MGC21336; MAGE-4 antigen; MAGE-41 antigen; MAGE-X2 antigen; cancer/testis antigen 1.4; cancer/testis antigen family 1, member 4; MAGE-41; MAGE-X2
• Gene ID: 4103
• mRNA Refseq: NM_001011548
• Protein Refseq: NP_001011548
• MIM: 300175
• UniProt ID: P43358