Recombinant Human MAPKAPK2 protein, His-tagged

• Cat.No.: MAPKAPK2-61H
• Product Overview: Recombinant Human MAPKAPK2 protein(1-400 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 1-400 aa
• AA Sequence: MLSNSQGQSPPVPFPAPAPPPQPPT PALPHPPAQPPPPPPQQFPQFHVKS GLQIKKNAIIDDYKVTSQVLGLGIN GKVLQIFNKRTQEKFALKMLQDCPK ARREVELHWRASQCPHIVRIVDVYE NLYAGRKCLLIVMECLDGGELFSRI QDRGDQAFTEREASEIMKSIGEAIQ YLHSINIAHRDVKPENLLYTSKRPN AILKLTDFGFAKETTSHNSLTTPCY TPYYVAPEVLGPEKYDKSCDMWSLG VIMYILLCGYPPFYSNHGLAISPGM KTRIRMGQYEFPNPEWSEVSEEVKM LIRNLLKTEPTQRMTITEFMNHPWI MQSTKVPQTPLHTSRVLKEDKERWE DVKEEMTSALATMRVDYEQIKIKKI EDASNPLLLKRRKKARALEAAALAH
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 [ Homo sapiens ]
• Official Symbol: MAPKAPK2
• Synonyms: MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MAP kinase-activated protein kinase 2; MAPKAP kinase 2; MAPK-activated protein kinase 2; MK2; MK-2; MAPKAP-K2
• Gene ID: 9261
• mRNA Refseq: NM_004759
• Protein Refseq: NP_004750
• MIM: 602006
• UniProt ID: P49137

Reviews

04/15/2022

The lab product sets a new standard for quality. Delivery was speedy, and the customer service team was exceptionally helpful.

11/23/2020

Reliable product, friendly customer service, and efficient delivery.

08/06/2019

Precision in quality, responsive customer service, and fast, secure delivery

Q&As

What structural features of MAPKAPK2 are critical for its kinase activity, and how do these features influence its interactions with substrates and regulatory partners?

MAPKAPK2's kinase activity is influenced by its structural features, including the catalytic domain. These features determine substrate interactions and regulatory partnerships, providing insights into its molecular functions.

What is known about the role of MAPKAPK2 in the immune response, particularly in the regulation of cytokine production and immune cell functions?

MAPKAPK2 plays a role in the immune response by regulating cytokine production and immune cell functions. It influences the activity of transcription factors involved in immune-related gene expression.

How does MAPKAPK2 contribute to cellular motility, and what molecular mechanisms are involved in its regulation of the cytoskeleton and cell migration?

MAPKAPK2 regulates cellular motility by influencing the cytoskeleton and cell migration. It phosphorylates proteins involved in cytoskeletal dynamics, impacting cell motility and invasive behavior.

Can you elaborate on the regulatory mechanisms controlling MAPKAPK2 activation and its substrate specificity?

MAPKAPK2 activation is regulated by p38 MAP kinase through phosphorylation. Its substrate specificity is determined by specific phosphorylation motifs, and its activity is influenced by interacting proteins.

In cancer research, how is MAPKAPK2 implicated in tumor development and progression, and are there potential therapeutic strategies targeting this kinase?

MAPKAPK2 has been associated with tumor development and progression. Understanding its role in cancer biology may lead to the development of targeted therapies, although challenges exist in achieving specificity.