Recombinant Human MASP1, His-tagged

• Cat.No.: MASP1-754H
• Product Overview: Recombinant Human MASP1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 29-321aa
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: MASP1?mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) [?Homo sapiens?(human) ]
• Official Symbol: MASP1
• Synonyms: MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1; mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor); mannan-binding lectin serine protease 1; serine protease 5; complement factor MASP-3; Ra-reactive factor serine protease p100; mannose-binding protein-associated serine protease; mannose-binding lectin-associated serine protease 1; complement-activating component of Ra-reactive factor; EC 3.4.21.-
• Gene ID: 5648
• mRNA Refseq: NM_001031849
• Protein Refseq: NP_001027019
• MIM: 600521
• UniProt ID: P48740
• Chromosome Location: 3q27-q28
• Pathway: Binding and Uptake of Ligands by Scavenger Receptors; Complement Activation; Complement and coagulation cascades
• Function: calcium ion binding; calcium-dependent protein binding; peptidase activity

Citation

AmpliSeq Screening of Genes Encoding the C-Type Lectin Receptors and Their Signaling Components Reveals a Common Variant in MASP1 Associated with Pulmonary Tuberculosis in an Indian Population

Journal: Frontiers in Immunology    PubMed ID: 29515573    Data: 2018/2/20

Authors: Tilman E. Klassert, Surabhi Goyal, Hortense Slevogt

Article Snippet:The serum MASP1 and MBL levels were measured by ELISA (Human MASP-1 ELISA Kit, Cloud-Clone Corp., Hycult Biotech Human MBL ELISA kit).The serum MASP1 and MBL levels were measured by ELISA (Human MASP-1 ELISA Kit, Cloud-Clone Corp., Hycult Biotech Human MBL ELISA kit).. To investigate the effect of increased MASP1 levels on complement function, recombinant human MASP1 (Creative BioMart MASP1-137H) was added at different concentrations (+13% rhMASP1, +26% rhMASP1, or +52% rhMASP1) to the donor serum, and complement activity after MBL pathway activation was measured using a commercially available ELISA kit (Complement system MBL pathway WIESLAB ? ) following manufacturer’s instructions.. Briefly, six diluted serum samples were measured in duplicate along with blank, positive and negative controls, and incubated at 37°C for 1 h. After washing, the formation of terminal complement complex C5b-9 was detected using conjugated antibody and absorbance was measured at 405 nm on a microplate reader (TECAN SpectraFluor Plus).Briefly, six diluted serum samples were measured in duplicate along with blank, positive and negative controls, and incubated at 37°C for 1 h. After washing, the formation of terminal complement complex C5b-9 was detected using..

List of common variants subjected to validation through MassARRAY, showing the results of the association analysis after phase II, as well as the joint analysis performed integrating data from phases I and II.

Distribution of allele and genotype frequencies for SNP rs3774275 ( MASP1 ) between controls and tuberculosis patients.

(A) Measurement of MASP1, MASP3, and MAp44 levels in serum of healthy controls and tuberculosis patients. Results after adjusting for BMI show significantly higher levels of MASP1 in tuberculosis patients ( T -test; ** p < 0.01) when compared to healthy controls. (B) Genotype-dependent distribution of MASP1, MASP3, and MAp44 levels across control and case samples. Shown are median values and 25–75th percentile box plots.