Recombinant Human MASP2 protein, His-tagged

• Cat.No.: MASP2-5432H
• Product Overview: Recombinant Human MASP2 protein(O00187-1)(Gly288-Phe686), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gly288-Phe686
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in 20mM Tris, 200mM NaCl (pH 9.0).
• Bio-activity: Immobilized Human MASP2, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 46.9 kDa. Due to Autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
• Storage: Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Concentration: Centrifuge tubes before opening.

Gene Information

• Gene Name: MASP2 mannan-binding lectin serine peptidase 2 [ Homo sapiens ]
• Official Symbol: MASP2
• Synonyms: MASP2; mannan-binding lectin serine peptidase 2; mannan binding lectin serine peptidase 1 pseudogene 1 , mannan binding lectin serine protease 1 pseudogene 1 , mannan binding lectin serine protease 2 , MASP1P1; mannan-binding lectin serine protease 2; small MBL-associated protein; MBL-associated serine protease 2; MBL-associated plasma protein of 19 kD; mannan-binding lectin serine protease 1 pseudogene 1; mannose-binding protein-associated serine protease 2; mannan-binding lectin serine peptidase 1 pseudogene 1; sMAP; MAP19; MASP-2; MASP1P1
• Gene ID: 10747
• mRNA Refseq: NM_006610
• Protein Refseq: NP_006601
• MIM: 605102
• UniProt ID: O00187