Active Recombinant Human MELTF protein, His-tagged, Low Endotoxin

• Cat.No.: MELTF-215H
• Product Overview: Recombinant Human MELTF protein(P08582-1)(Asp358-Cys709), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 358-709 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD228 Domain, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD228 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.