Active Recombinant Human MET Protein, His-Fc-tagged

• Cat.No.: MET-219H
• Product Overview: Active Recombinant Human MET Protein(Met 1-Thr 932)(NP_000236) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-932 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM.
• Molecular Mass: The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; HGFR; RCCP2; SF receptor; HGF receptor; oncogene MET; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; AUTS9; c-Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581