| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
2-395aa (full length) |
| Description : |
MAT2A (Methionine Adenosyltransferase 2A), also known as AMS2 or MATA2, is the primary extrahepatic isoform of S-adenosylmethionine (SAM) synthase. It catalyzes the formation of S-adenosyl-L-methionine (AdoMet/SAM) from L-methionine and ATP. MAT2A forms homo-dimers or homo-tetramers and can associate with the regulatory subunit MAT2B. The enzyme is central to one-carbon metabolism and methylation reactions. MAT2A is upregulated in hepatocellular carcinoma and other cancers, making it a key therapeutic target. EC 2.5.1.6. |
| Form : |
Liquid |
| Bio-activity : |
Specific activity > 85 pmol/min/μg, measured via hydrolysis of ATP during synthesis of S-adenosylmethionine (SAM) at 37°C. |
| Molecular Mass : |
44 kDa (2-395aa, His-tagged, theoretical); 48-55 kDa observed by SDS-PAGE (reducing conditions) |
| AASequence : |
MNGQLNGFHEAFIEEGTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDETDIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY |
| Endotoxin : |
< 0.10 EU/μg (LAL method) |
| Purity : |
> 95% (by SDS-PAGE, Silver Staining; quantitative densitometry by Coomassie Blue Staining) |
| Applications : |
Enzyme Activity |
| Storage : |
-20°C to -70°C; stable for 6 months from date of receipt (unopened); 3 months after opening under sterile conditions. Avoid freeze-thaw cycles. |
| Reaction conditions : |
37°C, pH 7.4 |
| EC : |
2.5.1.6 |
| CAS : |
9014-22-6 |
| Unit definition : |
One unit is defined as the amount of enzyme that hydrolyzes 1 pmol ATP per minute during synthesis of S-adenosyl-L-methionine at 37°C. |
| Storage Buffer : |
Tris, NaCl, TCEP and Glycerol (0.2 μm filtered) |
