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Recombinant Human METTL16 Protein, His-tagged

Cat.No. : METTL16-032H
Product Overview : Human recombinant C-terminal histidine-tagged human METTL16 protein (2-562) was purified from E. coli.
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  • Gene Information
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Description : RNA N6-methyltransferase that methylates adenosine residues at the N6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 64.5 kDa
Protein length : 2-562
Purity : ≥90% as determined by SDS-PAGE
Stability : ≥ 6 months
Storage : At -80 centigrade.
Storage Buffer : 50 mM HEPES, pH 8.0, with 150 mM sodium chloride, 10% glycerol
Gene Name : METTL16 methyltransferase 16, N6-methyladenosine [ Homo sapiens (human) ]
Official Symbol : METTL16
Synonyms : METTL16; methyltransferase 16, N6-methyladenosine; RNA N6-adenosine-methyltransferase METTL16; N6-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase; U6 snRNA methyltransferase; methyltransferase 10 domain containing; methyltransferase 10 domain-containing protein; methyltransferase like 16; methyltransferase-like protein 16; putative methyltransferase METT10D; EC 2.1.1.346; EC 2.1.1.348
Gene ID : 79066
mRNA Refseq : NM_024086
Protein Refseq : NP_076991
UniProt ID : Q86W50

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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