Active Recombinant Human MFAP2 protein, His-tagged

• Cat.No.: MFAP2-343H
• Product Overview: Active Recombinant Human MFAP2 protein(6-162 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 6-162 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose.
• Bio-activity: Microfibrillar-associated protein 2 (MFAP2) is an O-glycosylated protein which excreted to the extracellular space and the extracellular matrix. MFAP2 combine biglycan and elastin to form a ternary complex. MFAP2 plays a key role in the support and distensibility of the juxtacanalicular region of these collector channels. It also can inhibit LTB-1 binding to fibrillin-1, stimulate the phosphorylation of Smad2, and thereby mediate the subsequent extracellular deposition of latent TGFbeta. Besides, Fibrillin 1 (FBN1) has been identified as an interactor of MFAP2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MFAP2 and recombinant human FBN1. Briefly, MFAP2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to FBN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-MFAP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of MFAP2 and FBN1 was shown in Figure, and this effect was in a dose dependent manner.
• Molecular Mass: 22.0kDa
• Purity: >95%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: MFAP2 microfibrillar-associated protein 2 [ Homo sapiens ]
• Official Symbol: MFAP2
• Synonyms: MFAP2; microfibrillar-associated protein 2; MAGP; MAGP 1; microfibril-associated glycoprotein 1; MAGP1; MAGP-1; FLJ50901
• Gene ID: 4237
• mRNA Refseq: NM_001135247
• Protein Refseq: NP_001128719
• MIM: 156790
• UniProt ID: P55001