Recombinant Human MGP, His-tagged

• Cat.No.: MGP-3641H
• Product Overview: Recombinant human MGP protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process (20-96aa).

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: MGP, also known as Matrix Gla protein, is a protein involved in regulating calcification in the extracellular matrix, in particular in cartilage and arteries. MGP is a vitamin K-dependent protein, and contains five to six residues of Gla, a Ca2+ binding amino acid requiring vitamin K-dependent gamma carboxylase for its formation. It is found in human bone extracts, likely formed by COOH-terminal processing by carboxypeptidase B-like enzymatic activity.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
• Molecular Weight: 11.8 kDa (98aa)
• Purity: > 90% by SDS - PAGE
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MYESHESMES YELNPFINRR NANTFISPQQ RWRAKVQERI RERSKPVHEL NREACDDYRL CERYAMVYGY NAAYNRYF
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Endochondral Ossification; Validated transcriptional targets of AP1 family members Fra1 and Fra2

Gene Information

• Gene Name: MGP matrix Gla protein [ Homo sapiens ]
• Official Symbol: MGP
• Synonyms: MGP; NTI; GIG36; MGLAP; matrix Gla protein; OTTHUMP00000239335; OTTHUMP00000239337; cell growth-inhibiting gene 36 protein
• Gene ID: 4256
• mRNA Refseq: NM_000900
• Protein Refseq: NP_000891
• MIM: 154870
• UniProt ID: P08493
• Chromosome Location: 12p12.3
• Function: calcium ion binding; extracellular matrix structural constituent; structural constituent of bone