|Product Overview:||Recombinant Human MIA (Gly25-Gln131) fused with Met at N-terminal was expressed in E. coli.|
|Description:||Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is secreted as a noncovalent homodimer and is structurally related to OTOR/Otoraplin and MIA-2. Mature human MIA contains a SH3 domain and shares 90% and 92% amino acid sequence identity with mouse and rat MIA, respectively. Alternative splicing generates a short isoform that lacks the SH3 domain. MIA is widely expressed in developing and regenerating cartilage and in the endothelium and parenchyma of developing lungs. MIA disrupts cellular interactions with the extracellular matrix by binding to Integrins alpha 4 beta 1 and alpha 5 beta 1. It competes with Fibronectin fragments for Integrin binding and interferes with Integrin signaling. It also functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta 3 induced differentiation into chondrocytes [tscheud]. MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair. MIA is up-regulated in several cancers including malignant melanoma, lung adenoma, metastatic oral squamous cell carcinoma, neurofibromatosis type 1 (NF-1)-related tumors, and pancreatic cancer. It is selectively secreted and internalized from the trailing pole of migrating cells. This polarization reduces cellular attachment to the matrix at the trailing pole and contributes to directional tumor cell migration.|
|Predicted N Terminal:||Met, Gly25|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.|
|Bio-activity:||Measured by its binding ability in a functional ELISA. When Recombinant Human Fibronectin Fragment 4 is immubilized at 1 μg/mL, 100 μL/well, Recombinant Human MIA binds with a typical ED50 of 1-6 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 12 kDa
SDS-PAGE: 10 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 400 μg/mL in PBS.|
|Gene Name:||MIA melanoma inhibitory activity [ Homo sapiens ]|
|Synonyms:||MIA; melanoma inhibitory activity; melanoma-derived growth regulatory protein; CD RAP; CD-RAP;|
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