Active Recombinant Human MICB protein, His-Avi-tagged, Low Endotoxin

• Cat.No.: MICB-980H
• Product Overview: Recombinant Human MICB protein(Q29980-1)(23-298 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 23-298 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human MICB, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MICB MHC class I polypeptide-related sequence B [ Homo sapiens ]
• Official Symbol: MICB
• Synonyms: MICB; MHC class I polypeptide-related sequence B; PERB11.2; MIC-B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• Gene ID: 4277
• mRNA Refseq: NM_005931
• Protein Refseq: NP_005922
• MIM: 602436
• UniProt ID: Q29980