Recombinant Human MIS12 protein, His-tagged

• Cat.No.: MIS12-7228H
• Product Overview: Recombinant Human MIS12 protein(1-205 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-205 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AASequence: MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: MIS12 MIS12, MIND kinetochore complex component, homolog (S. pombe) [ Homo sapiens ]
• Official Symbol: MIS12
• Synonyms: MIS12; MIS12, MIND kinetochore complex component, homolog (S. pombe); MIS12, MIND kinetochore complex component, homolog (yeast); protein MIS12 homolog; hMIS12; KNTC2AP; MGC2488; MTW1; MIS12 homolog; homolog of yeast Mis12; hMis12; 2510025F08Rik
• Gene ID: 79003
• mRNA Refseq: NM_001258217
• Protein Refseq: NP_001245146
• MIM: 609178
• UniProt ID: Q9H081