Active Recombinant Human MKNK1 Protein, GST-tagged

• Cat.No.: MKNK1-5365H
• Product Overview: Human MKNK1 (BAA19885.1, 1 a.a. - 424 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate:RS peptide.
  ATP: 100 μM.
• Molecular Mass: 74 kDa
• Purity: 93 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: MKNK1 MAP kinase interacting serine/threonine kinase 1 [ Homo sapiens ]
• Official Symbol: MKNK1
• Synonyms: MKNK1; MAP kinase interacting serine/threonine kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MNK1; MAPK signal-integrating kinase 1; MAP kinase signal-integrating kinase 1
• Gene ID: 8569
• mRNA Refseq: NM_001135553
• Protein Refseq: NP_001129025
• MIM: 606724
• UniProt ID: Q9BUB5