Recombinant Human MKNK1 Protein, GST-tagged

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Recombinant Human MKNK1 Protein, GST-tagged

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Cat.No. : MKNK1-5365H
Product Overview : Human MKNK1 (BAA19885.1, 1 a.a. - 424 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
Source : Insect cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate:RS peptide.
ATP: 100 μM.
Molecular Mass : 74 kDa
Purity : 93 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Quality Control Testing: :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : MKNK1 MAP kinase interacting serine/threonine kinase 1 [ Homo sapiens ]
Official Symbol : MKNK1
Synonyms : MKNK1; MAP kinase interacting serine/threonine kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MNK1; MAPK signal-integrating kinase 1; MAP kinase signal-integrating kinase 1;
Gene ID : 8569
mRNA Refseq : NM_001135553
Protein Refseq : NP_001129025
MIM : 606724
UniProt ID : Q9BUB5

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