Recombinant Human Mononucleosome (H3.1ΔN2), Biotinylated

• Cat.No.: NUC-007
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1ΔN2 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) with the amino acid sequence of H3 beginning with threonine 3, wrapped by 147 base pairs of 601 positioning sequence DNA. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group.
• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated (27.3 μg protein weight, 50 μg DNA+protein) in μl 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 199,405.26 Da
• Applications: Mononucleosome (H3.1ΔN2), Recombinant Human, Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays, structural studies, or for effector protein binding experiments. The N-terminal deletion allows for the study of the role of the N-terminus in many aspects of chromatin biology.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.