Recombinant Human Mononucleosomes, Desthiobiotinylated

• Cat.No.: NUC-005
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped with a 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (Kd = 10-11) for avidin-family proteins than does biotin (Kd = 10-15).
• Form: Purified recombinant mononucleosomes (50 µg total mass, 27.3 µg protein + 22.7 µg DNA in 42.5 µL) in 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 199,827.86 Da
• Applications: Mononucleosome, Recombinant Human, Desthiobiotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The weaker affinity of desthiobiotin for avidin-family proteins allows nucleosomes to be eluted via addition of free biotin. The ability of these nucleosomnes to be recovered intact is useful in many aspects of chromatin biology
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
• Conjugation: Biotin