Recombinant Human Mononucleosomes, Desthiobiotinylated
Cat.No. : | NUC-005 |
Product Overview : | Mononucleosomes assembled from recombinant human histones expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.1 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped with a 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' desthiobiotin-TEG group. Desthiobiotin has a weaker affinity (Kd = 10-11) for avidin-family proteins than does biotin (Kd = 10-15). |
Source : | E. coli |
Species : | Human |
Tag : | N/A |
Form : | Purified recombinant mononucleosomes (50 µg total mass, 27.3 µg protein + 22.7 µg DNA in 42.5 µL) in 10 mM Tris-HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. |
Molecular Mass : | 199,827.86 Da |
Applications : | Mononucleosome, Recombinant Human, Desthiobiotinylated are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The weaker affinity of desthiobiotin for avidin-family proteins allows nucleosomes to be eluted via addition of free biotin. The ability of these nucleosomnes to be recovered intact is useful in many aspects of chromatin biology |
Storage : | Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws. |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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