Recombinant Human Mononucleosomes (H2AX), Biotinylated

• Cat.No.: NUC-037
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2AX, H2B, H3 and H4. Accession numbers: H2AX-P16104; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA with a 5’ biotin-TEG group. The nucleosome is the basic subunit of chromatin. H2AX is a variant of histone H2A that is phosphorylated on serine 139 by the ATM and ATR kinases in response to DNA double strand breakage. Phosphorylation of H2AX serves to recruit a number of DNA damage repair proteins, such as BRCA1 and NBS1.
• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Purified recombinant mononucleosomes (50 µg total mass, 27.4 µg in 50.0 µl) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT and 20% glycerol. Concentration of nucleosomes is 4.94 µM.
• Molecular Mass: 202,308.2 Da
• Applications: Mononucleosomes (H2AX), Human Recombinant Biotinylated are highly purified and are suitable for use as substrates in enzyme screening assays or for nucleosome binding experiments. The absence of post-translational histone modifications makes them ideal for conducting enzyme activity and screening assays. Mononucleosomes (H2AX), Human Recombinant Biotinylated do not contain free DNA which could alter assayed activities.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.