Recombinant Human Mononucleosomes (H3.3 ΔN32)

• Cat.No.: NUC-010
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3.3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.3-P84243; H4-P62805), with the amino acid sequence of H3 begining with glycine 33 (amino acids 1-32 are deleted), wrapped by 147 base pairs of 601 positioning sequence DNA. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly.
• Form: Mononucleosomes (H3.3 ΔN32), Recombinant Human (27.4 µg protein weight, 50 µg DNA+protein) in 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol.
• Molecular Mass: 193,766.7 Da
• Applications: Mononucleosomes (H3.3 ΔN32), Human Recombinant are highly purified and suitable for use as substrates in enzyme screening assays, structural studies, or effector protein binding experiments. The N-terminal deletion allows for the study of the role of the N-terminus in many aspects of chromatin biology.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.