Recombinant Human MSH3 HEK293T cell lysate

• Cat.No.: MSH3-23HCL
• Product Overview: Human MSH3 was derived in Human HEK293T cell line.

Specification

• Description: The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.
• Source: HEK293
• Species: Human
• Tag: C-terminal Myc/DDK
• Form: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Molecular Mass: 127.2 kDa
• Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: MSH3 mutS homolog 3 (E. coli) [ Homo sapiens ]
• Official Symbol: MSH3
• Synonyms: MSH3; mutS homolog 3 (E. coli); mutS (E. coli) homolog 3; DNA mismatch repair protein Msh3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1; hMSH3; mismatch repair protein 1; divergent upstream protein; MGC163306; MGC163308
• Gene ID: 4437
• mRNA Refseq: NM_002439
• Protein Refseq: NP_002430
• MIM: 600887
• UniProt ID: P20585