Recombinant Human MYL1 protein, His-tagged

• Cat.No.: MYL1-1507H
• Product Overview: Recombinant Human MYL1 aa. (Phe3~Ala142) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Phe3~Ala142
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 4.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
  Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: MYL1 myosin light chain 1 [ Homo sapiens (human) ]
• Official Symbol: MYL1
• Synonyms: MLC1F; MLC3F; A1 catalytic; A2 catalytic; MLC1/MLC3; MLC1F/MLC3F; myosin light chain A1/A2; myosin light chain alkali 1/2; myosin, light chain 1, alkali; skeletal, fast; myosin, light polypeptide 1, alkali; skeletal, fast; myosin light chain 1/3, skeletal muscle isoform
• Gene ID: 4632
• mRNA Refseq: NM_079420.2
• Protein Refseq: NP_524144.1
• UniProt ID: P05976