Recombinant Human N-acetylneuraminic Acid Phosphatase, His-tagged

• Cat.No.: NANP-2825H
• Product Overview: Recombinant human NANP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 31.9kDa (284aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating N-acylneuraminate 9-phosphate to form N-acylneuraminate (N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate). Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium.
• Sequences Of Amino Acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGLS RVRAVFFDLD NTLIDTAGAS RRGMLEVIKL LQSKYHYKEE AEIICDKVQV KLSKECFHPY NTCITDLRTS HWEEAIQETK GGAANRKLAE ECYFLWKSTR LQHMTLAEDV KAMLTELRKE VRLLLLTNGD RQTQREKIEA CACQSYFDAV VVGGEQREEK PAPSIFYYCC NLLGVQPGDC VMVGDTLETD IQGGLNAGLK ATVWINKNGI VPLKSSPVPH YMVSSVLELP ALLQSIDCKV SMST
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NANP N-acetylneuraminic acid phosphatase [ Homo sapiens ]
• Synonyms: NANP; N-acetylneuraminic acid phosphatase; HDHD4; MGC26833; C20orf147; dJ694B14.3; N-acylneuraminate-9-phosphatase; Neu5Ac-9-Pase; haloacid dehalogenase-like hydrolase domain containing 4; haloacid dehalogenase-like hydrolase domain-containing protein 4; EC 3.1.3.29; chromosome 20 open reading frame 147
• Gene ID: 140838
• mRNA Refseq: NM_152667
• Protein Refseq: NP_689880
• MIM: 610763
• UniProt ID: Q8TBE9
• Chromosome Location: 20p11.1
• Pathway: Amino sugar and nucleotide sugar metabolism; Metabolic pathways
• Function: N-acylneuraminate-9-phosphatase activity; hydrolase activity; phosphoglycolate phosphatase activity