Recombinant Human N(alpha)-acetyltransferase 30, NatC catalytic subunit, His-tagged

• Cat.No.: NAA30-226H
• Product Overview: NAA30, 1-362aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-362 a.a.
• Description: N-alpha-acetyltransferase 30, also known as NAA30, is catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. This protein catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. This protein is necessary for the lysosomal localization and function of ARL8B. Recombinant human NAA30 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 41.7 kDa (385aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAEVPPG PSSLLPPPAP PAPAAVEPRC PFPAGAALAC CSEDEEDDEE HEGGGSRSPA GGESATVAAK GHPCLRCPQP PQEQQQLNGL ISPELRHLRA AASLKSKVLS VAEVAATTAT PDGGPRATAT KGAGVHSGER PPHSLSSNAR TAVPSPVEAA AASDPAAARN GLAEGTEQEE EEEDEQVRLL SSSLTADCSL RSPSGREVEP GEDRTIRYVR YESELQMPDI MRLITKDLSE PYSIYTYRYF IHNWPQLCFL AMVGEECVGA IVCKLDMHKK MFRRGYIAML AVDSKYRRNG IGTNLVKKAI YAMVEGDCDE VVLETEITNK SALKLYENLG FVRDKRLFRY YLNGVDALRL KLWLR
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NAA30 N(alpha)-acetyltransferase 30, NatC catalytic subunit [ Homo sapiens ]
• Official Symbol: NAA30
• Synonyms: NAA30; N(alpha)-acetyltransferase 30, NatC catalytic subunit; C14orf35, chromosome 14 open reading frame 35 , N acetyltransferase 12 , N acetyltransferase 12 (GCN5 related, putative) , NAT12; N-alpha-acetyltransferase 30; FLJ35355; MAK3; Mak3p; natC catalytic subunit; putative N-acetyltransferase; N-acetyltransferase MAK3 homolog; N-acetyltransferase 12 (GCN5-related, putative); N-alpha-acetyltransferase 30, NatC catalytic subunit; NAT12; C14orf35; MGC141884; MGC141885
• Gene ID: 122830
• mRNA Refseq: NM_001011713
• Protein Refseq: NP_001011713
• UniProt ID: Q147X3
• Chromosome Location: 14q22.2
• Function: N-acetyltransferase activity; peptide alpha-N-acetyltransferase activity; transferase activity, transferring acyl groups