Recombinant Human NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 4, His-tagged

• Cat.No.: NDUFAF4-7199H
• Product Overview: Recombinant human NDUFAF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 22.7 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-175aa
• Description: DH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4, also known as NDUFAF4, is involved in the assembly of mitochondrial NADH: ubiquinone oxidoreductase complex (complex I). This protein may be involved in cell proliferation and survival of hormone-dependent tumor cells. This protein may be a regulator of breast tumor cell invasion.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 22.7 kDa (198aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGALVIR GIRNFNLENR AEREISKMKP SVAPRHPSTN SLLREQISLY PEVKGEIARK DEKLLSFLKD VYVDSKDPVS SLQVKAAETC QEPKEFRLPK DHHFDMINIK SIPKGKISIV EALTLLNNHK LFPETWTAEK IMQEYQLEQK DVNSLLKYFV TFEVEIFPPE DKKAIRSK
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NDUFAF4 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4 [ Homo sapiens ]
• Official Symbol: NDUFAF4
• Synonyms: NDUFAF4; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4; C6orf66, chromosome 6 open reading frame 66; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4; bA22L21.1; HRPAP20; HSPC125; My013; hormone-regulated proliferation-associated protein, 20 kDa; hormone-regulated proliferation-associated protein of 20 kDa; C6orf66
• Gene ID: 29078
• mRNA Refseq: NM_014165
• Protein Refseq: NP_054884
• MIM: 611776
• UniProt ID: Q9P032
• Chromosome Location: 6q16.3
• Function: calmodulin binding; protein binding