Recombinant Human NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase), His-tagged

• Cat.No.: NDUFS3-346H
• Product Overview: NDUFS3, 37-264aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 37-264 a.a.
• Description: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, also known as NDUFS3, belongs to the complex I 30 kDa subunit family. The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits. Recombinant human NDUFS3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 28.7 kDa (249aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MESAGADTRP TVRPRNDVAH KQLSAFGEYV AEILPKYVQQ VQVSCFNELE VCIHPDGVIP VLTFLRDHTN AQFKSLVDLT AVDVPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPIES AVSVFKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG HPFRKDFPLS GYVELRYDDE VKRVVAEPVE LAQEFRKFDL NSPWEAFPVY RQPPESLKLE AGDKKPDAK
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NDUFS3 NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase) [ Homo sapiens ]
• Official Symbol: NDUFS3
• Synonyms: NDUFS3; NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase); NADH dehydrogenase (ubiquinone) Fe S protein 3 (30kD) (NADH coenzyme Q reductase); NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; CI 30; complex I 30kDa subunit; NADH dehydrogenase [ubiquinone] iron sulfur protein 3; mitochondrial; CI-30kD; complex I-30kD; NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit; CI-30
• Gene ID: 4722
• mRNA Refseq: NM_004551
• Protein Refseq: NP_004542
• MIM: 603846
• UniProt ID: O75489
• Chromosome Location: 11p11.11
• Pathway: 3-phosphoinositide degradation, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Electron Transport Chain, organism-specific biosystem; Huntingtons disease, organism-specific biosystem; Huntingtons disease, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity; electron carrier activity; protein binding