Recombinant Human NAMPT protein(Met1-His491), His&GST-tagged

• Cat.No.: NAMPT-2531H
• Product Overview: Recombinant Human NAMPT (P43490) (Met 1-His 491) was expressed in Insect Cells, fused with a N-terminal polyhistidine tag followed by a GST tag.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-His491
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 0.3mM DTT Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human NAMPT/GST chimera consists of 728 amino acids and has a calculated molecular mass of 83.3 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens ]
• Official Symbol: NAMPT
• Synonyms: NAMPT; nicotinamide phosphoribosyltransferase; PBEF1, pre B cell colony enhancing factor 1; PBEF; visfatin; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; VF; PBEF1; VISFATIN; 1110035O14Rik; MGC117256; DKFZp666B131
• Gene ID: 10135
• mRNA Refseq: NM_005746
• Protein Refseq: NP_005737
• MIM: 608764
• UniProt ID: P43490