Recombinant Human NCAM1 Protein, His-tagged

• Cat.No.: NCAM1-02H
• Product Overview: Recombinant human NCAM-1 (20-603aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms.
• Source: Insects cells
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 65.7kDa(593aa)
• AA Sequence: LQVDIVPSQGEISVGESKFFLCQVA GDAKDKDISWFSPNGEKLTPNQQRI SVVWNDDSSSTLTIYNANIDDAGIY KCVVTGEDGSESEATVNVKIFQKLM FKNAPTPQEFREGEDAVIVCDVVSS LPPTIIWKHKGRDVILKKDVRFIVL SNNYLQIRGIKKTDEGTYRCEGRIL ARGEINFKDIQVIVNVPPTIQARQN IVNATANLGQSVTLVCDAEGFPEPT MSWTKDGEQIEQEEDDEKYIFSDDS SQLTIKKVDKNDEAEYICIAENKAG EQDATIHLKVFAKPKITYVENQTAM ELEEQVTLTCEASGDPIPSITWRTS TRNISSEEKTLDGHMVVRSHARVSS LTLKSIQYTDAGEYICTASNTIGQD SQSMYLEVQYAPKLQGPVAVYTWEG NQVNITCEVFAYPSATISWFRDGQL LPSSNYSNIKIYNTPSASYLEVTPD SENDFGNYNCTAVNRIGQESLEFIL VQADTPSSPSIDQVEPYSSTAQVQF DEPEATGGVPILKYKAEWRAVGEEV WHSKWYDAKEASMEGIVTIVGLKPE TTYAVRLAALNGKGLGEISAASEFK TQPVHSPPP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: NCAM1 neural cell adhesion molecule 1 [ Homo sapiens (human) ]
• Official Symbol: NCAM1
• Synonyms: NCAM1; neural cell adhesion molecule 1; CD56; NCAM; MSK39; neural cell adhesion molecule 1; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule, NCAM; EC 2.7.11.1
• Gene ID: 4684
• mRNA Refseq: NM_001076682
• Protein Refseq: NP_001070150
• MIM: 116930
• UniProt ID: P13591