| Description : |
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
38.9 kDa |
| AA Sequence : |
MAVAQQLRAESDFEQLPDDVAISAN IADIEEKRGFTSHFVFVIEVKTKGG SKYLIYRRYRQFHALQSKLEERFGP DSKSSALACTLPTLPAKVYVGVKQE IAEMRIPALNAYMKSLLSLPVWVLM DEDVRIFFYQSPYDSEQVPQALRRL RPRTRKVKSVSPQGNSVDRMAAPRA EALFDFTGNSKLELNFKAGDVIFLL SRINKDWLEGTVRGATGIFPLSFVK ILKDFPEEDDPTNWLRCYYYEDTIS TIKDIAVEEDLSSTPLLKDLLELTR REFQREDIALNYRDAEGDLVRLLSD EDVALMVRQARGLPSQKRLFPWKLH ITQKDNYRVYNTMPTRTRPLEQKLI SEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
