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Active Recombinant Human NECTIN2 protein, Fc-tagged

Cat.No. : NECTIN2-233H
Product Overview : Recombinant Human NECTIN2(Gln32-Leu360) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.
  • Specification
  • Gene Information
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Description : Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. The 65 kDa long isoform of human Nectin-2/CD112 (Nectin-2δ) consists of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain. Within the ECD, human Nectin-2 shares 72% aa sequence identity with mouse Nectin-2. Alternative splicing generates a short 60 kDa isoform with a 94 aa cytoplasmic tail. Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts. It forms homodimers in cis, followed by dimers in trans (between cells). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell. Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity. Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus. Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload.
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : No results obtained: Gln32 predicted
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin‑2/CD112 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human DNAM‑1 Fc Chimera that produces 50% of the optimal binding response is 0.04-0.2 μg/mL.
Molecular Mass : Predicted Molecular Mass: 62 kDa
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
Gene Name : NECTIN2 nectin cell adhesion molecule 2 [ Homo sapiens ]
Official Symbol : NECTIN2
Synonyms : HVEB; PRR2; CD112; PVRL2; PVRR2
Gene ID : 5819
mRNA Refseq : NM_001042724
Protein Refseq : NP_001036189
MIM : 600798
UniProt ID : Q92692

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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