|Product Overview:||Recombinant Human NECTIN2(Gln32-Leu360) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.|
|Description:||Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. The 65 kDa long isoform of human Nectin-2/CD112 (Nectin-2δ) consists of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain. Within the ECD, human Nectin-2 shares 72% aa sequence identity with mouse Nectin-2. Alternative splicing generates a short 60 kDa isoform with a 94 aa cytoplasmic tail. Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts. It forms homodimers in cis, followed by dimers in trans (between cells). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell. Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity. Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus. Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload.|
|Predicted N Terminal:||No results obtained: Gln32 predicted|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin‑2/CD112 Fc Chimera is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human DNAM‑1 Fc Chimera that produces 50% of the optimal binding response is 0.04-0.2 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 62 kDa|
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||NECTIN2 nectin cell adhesion molecule 2 [ Homo sapiens ]|
|Synonyms:||HVEB; PRR2; CD112; PVRL2; PVRR2|
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