Recombinant Human NECTIN4 protein, His-Avi-tagged, Biotinylated

• Cat.No.: NECTIN4-968HB
• Product Overview: Biotinylated Recombinant Human NECTIN4 protein(Q96NY8)(Gly32-Val351), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly32-Val351
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.