Recombinant Human NEK2, Active

• Cat.No.: NEK2-663H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C, and its catalytic domain is most similar to protein kinase B (PKB). SGK2, like the other two isoforms SGK1 and SGK3, is stimulated by insulin and insulin-like growth factor-1 (IGF-1), and has been shown to enhance Na(+)/K(+)-ATPase activity in a variety of cells.
• Application: Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
• Biochem/physiol Actions: Nek2 (nimA-related kinase 2) is a cell cycle-regulated protein kinase with maximal activity at the onset of mitosis that localizes to the centrosome.
• Physical Form: Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
• Assay: ≥85% (SDS-PAGE).
• Form: buffered aqueous glycerol solution.
• Mol Wt: protein apparent mol wt ~80 kDa.
• Storage Temp: −70°C.

Gene Information

• Gene Name: NEK2 NIMA (never in mitosis gene a)-related kinase 2 [ Homo sapiens ]
• Synonyms: NEK2; NIMA (never in mitosis gene a)-related kinase 2; NLK1; NEK2A; HsPK21; NIMA-related kinase 2; EC 2.7.11.1; serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; never in mitosis A-related kinase 2
• Gene ID: 4751
• mRNA Refseq: NM_002497
• Protein Refseq: NP_002488
• MIM: 604043
• UniProt ID: P51955
• Chromosome Location: 1q32.2-q41
• Pathway: Cell Cycle, Mitotic
• Function: ATP binding; magnesium ion binding; transferase activity; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity