Recombinant Human NEU1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NEU1-5667H
• Product Overview: NEU1 MS Standard C13 and N15-labeled recombinant protein (NP_000425) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a lysosomal enzyme that cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter is also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis, a lysosomal storage disease that can be type 1 (cherry red spot-myoclonus syndrome or normosomatic type), which is late-onset, or type 2 (the dysmorphic type), which occurs at an earlier age with increased severity.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 45.5 kDa
• AA Sequence: MTGERPSTALPDRRWGPRILGFWGG CRVWVFAAIFLLLSLAASWSKAEND FGLVQPLVTMEQLLWVSGRQIGSVD TFRIPLITATPRGTLLAFAEARKMS SSDEGAKFIALRRSMDQGSTWSPTA FIVNDGDVPDGLNLGAVVSDVETGV VFLFYSLCAHKAGCQVASTMLVWSK DDGVSWSTPRNLSLDIGTEVFAPGP GSGIQKQREPRKGRLIVCGHGTLER DGVFCLLSDDHGASWRYGSGVSGIP YGQPKQENDFNPDECQPYELPDGSV VINARNQNNYHCHCRIVLRSYDACD TLRPRDVTFDPELVDPVVAAGAVVT SSGIVFFSNPAHPEFRVNLTLRWSF SNGTSWRKETVQLWPGPSGYSSLAT LEGSMDGEEQAPQLYVLYEKGRNHY TESISVAKISVYGTLTRTRPLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NEU1 neuraminidase 1 [ Homo sapiens (human) ]
• Official Symbol: NEU1
• Synonyms: NEU1; sialidase 1 (lysosomal sialidase); NEU; sialidase-1; G9 sialidase; exo-alpha-sialidase; lysosomal sialidase; acetylneuraminyl hydrolase; N-acetyl-alpha-neuraminidase 1; NANH; SIAL1; FLJ93471
• Gene ID: 4758
• mRNA Refseq: NM_000434
• Protein Refseq: NP_000425
• MIM: 608272
• UniProt ID: Q99519