Recombinant Human nicotinamide nucleotide adenylyltransferase 2, His-tagged

• Cat.No.: NMNAT2-300H
• Product Overview: NMNAT2, 1-307 aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-307 a.a.
• Description: NMNAT2 belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. Recombinant human NMNAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 36.6 kDa (327aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ NETPQPIYQN SNVATKPTAA KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ LYINASG
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: NMNAT2 nicotinamide nucleotide adenylyltransferase 2 [ Homo sapiens ]
• Official Symbol: NMNAT2
• Synonyms: NMNAT2; nicotinamide nucleotide adenylyltransferase 2; C1orf15, chromosome 1 open reading frame 15; nicotinamide mononucleotide adenylyltransferase 2; KIAA0479; PNAT2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2; pyridine nucleotide adenylyltransferase 2; nicotinate-nucleotide adenylyltransferase 2; C1orf15; MGC2756
• Gene ID: 23057
• mRNA Refseq: NM_015039
• Protein Refseq: NP_055854
• MIM: 608701
• UniProt ID: Q9BZQ4
• Chromosome Location: 1q25
• Pathway: Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem; NAD biosynthesis II (from tryptophan), organism-specific biosystem; NAD biosynthesis III, organism-specific biosystem; NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde, organism-specific biosystem
• Function: ATP binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; nucleotide binding; nucleotidyltransferase activity; transferase activity