Recombinant Human NOB1 Protein, GST-tagged

• Cat.No.: NOB1-5959H
• Product Overview: Human NOB1 full-length ORF ( NP_054781.1, 1 a.a. - 412 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 73.1 kDa
• AA Sequence: MAPVEHVVADAGAFLRHAALQDIGK NIYTIREVVTEIRDKATRRRLAVLP YELRFKEPLPEYVRLVTEFSKKTGD YPSLSATDIQVLALTYQLEAEFVGV SHLKQEPQKVKVSSSIQHPETPLHI SGFHLPYKPKPPQETEKGHSACEPE NLEFSSFMFWRNPLPNIDHELQELL IDRGEDVPSEEEEEEENGFEDRKDD SDDDGGGWITPSNIKQIQQELEQCD VPEDVRVGCLTTDFAMQNVLLQMGL HVLAVNGMLIREARSYILRCHGCFK TTSDMSRVFCSHCGNKTLKKVSVTV SDDGTLHMHFSRNPKVLNPRGLRYS LPTPKGGKYAINPHLTEDQRFPQLR LSQKARQKTNVFAPDYIAGVSPFVE NDISSRSATLQVRDSTLGAGRRRLN PNASRKKFVKKR
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: NOB1 NIN1/RPN12 binding protein 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: NOB1
• Synonyms: NOB1; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); PSMD8 binding protein 1 , PSMD8BP1; RNA-binding protein NOB1; ART 4; MST158; nin one binding protein; NOB1P; protein ART-4; PSMD8 binding protein 1; phosphorylation regulatory protein HP-10; adenocarcinoma antigen recognized by T lymphocytes 4; ART-4; MSTP158; PSMD8BP1
• Gene ID: 28987
• mRNA Refseq: NM_014062
• Protein Refseq: NP_054781
• MIM: 613586
• UniProt ID: Q9ULX3