Recombinant Human NOSTRIN protein, His & T7-tagged

• Cat.No.: NOSTRIN-630H
• Product Overview: Recombinant Human NOSTRIN aa. (Lys18~Ala251 (Accession # Q8IVI9)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.5kDa
• Protein length: Lys18~Ala251 (Accession # Q8IVI9)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: NOSTRIN nitric oxide synthase trafficking [ Homo sapiens (human) ]
• Official Symbol: NOSTRIN
• Synonyms: NOSTRIN; nitric oxide synthase trafficking; DaIP2; nostrin; BM247 homolog; ENOS traffic inducer; eNOS-trafficking inducer; endothelial nitric oxide synthase traffic inducer; nitric oxide synthase traffic inducer; nitric oxide synthase trafficker; ortholog of mouse disabled 2 interacting proein 2
• Gene ID: 115677
• mRNA Refseq: NM_001039724.3
• Protein Refseq: NP_001034813.2
• UniProt ID: Q8IVI9