Recombinant Human NOTCH1 protein, His-Avi-tagged

• Cat.No.: NOTCH1-531H
• Product Overview: Recombinant Human Notch 1 Protein is expressed from mammalian with His Tag and Avi Tag at the C-terminal. It contains Ala19-Gln526.

Specification

• Description: Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membranebound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 56.5 KDa. Due to glycosylation, the protein migrates to 70- 80KDa based on Bis-Tris PAGE result.
• Protein length: Ala19-Gln526
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.

Gene Information

• Gene Name: NOTCH1
• Official Symbol: NOTCH1
• Synonyms: EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
• Gene ID: 4851
• mRNA Refseq: NM_017617.5
• Protein Refseq: NP_060087.3
• MIM: 190198
• UniProt ID: P46531

Reviews

01/24/2021

Continuous access to up-to-date training materials ensured that our team remained well-versed in the product's capabilities.

05/09/2017

The product's evolution in response to user feedback showcased a dedication to meeting the evolving needs of researchers.

11/04/2016

The product's compatibility with open science practices positively influenced our decision to adopt it for our experiments.

Q&As

How does NOTCH1 contribute to the regulation of embryonic development and tissue homeostasis?

NOTCH1 contributes to the regulation of embryonic development and tissue homeostasis by influencing cell differentiation and proliferation.

What are the ligands or interacting partners of NOTCH1, and how do they regulate its activation?

The ligands or interacting partners of NOTCH1 include Delta-like 1 (DLL1), Delta-like 3 (DLL3), Delta-like 4 (DLL4), Jagged 1, and Jagged 2, regulating its activation.

What is the primary function of Neurogenic locus notch homolog protein 1 (NOTCH1) in cellular processes and signaling?

Neurogenic locus notch homolog protein 1 (NOTCH1) functions in cellular processes, primarily playing a role in the Notch signaling pathway.

What diseases or conditions are associated with dysregulation or abnormal expression of NOTCH1?

Dysregulation or abnormal expression of NOTCH1 is associated with conditions such as cancer, cardiovascular diseases, and developmental disorders.

In which cellular compartments is NOTCH1 predominantly localized?

NOTCH1 is predominantly localized on the cell membrane, where it undergoes proteolytic cleavage upon activation.

Are there therapeutic implications or potential targets related to NOTCH1 in diseases involving Notch signaling?

There are potential therapeutic implications related to NOTCH1 in diseases involving Notch signaling, making it a target for drug development.

How does NOTCH1 participate in the Notch signaling pathway, and what is its role in cell fate determination?

NOTCH1 participates in the Notch signaling pathway by interacting with ligands such as Delta and Jagged, influencing cell fate determination.