Recombinant Human NP Protein, GST-tagged

• Cat.No.: NP-6012H
• Product Overview: Human NP full-length ORF ( NP_000261.1, 1 a.a. - 289 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 58.5 kDa
• AA Sequence: MENGYTYEDYKNTAEWLLSHTKHRP QVAIICGSGLGGLTDKLTQAQIFDY GEIPNFPRSTVPGHAGRLVFGFLNG RACVMMQGRFHMYEGYPLWKVTFPV RVFHLLGVDTLVVTNAAGGLNPKFE VGDIMLIRDHINLPGFSGQNPLRGP NDERFGDRFPAMSDAYDRTMRQRAL STWKQMGEQRELQEGTYVMVAGPSF ETVAECRVLQKLGADAVGMSTVPEV IVARHCGLRVFGFSLITNKVIMDYE SLEKANHEEVLAAGKQAAQKLEQFV SILMASIPLPDKAS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: PNP purine nucleoside phosphorylase [ Homo sapiens (human) ]
• Official Symbol: NP
• Synonyms: PNP; purine nucleoside phosphorylase; NP; PUNP; PRO1837; purine nucleoside phosphorylase; HEL-S-156an; epididymis secretory sperm binding protein Li 156an; inosine phosphorylase; inosine-guanosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase; EC 2.4.2.1
• Gene ID: 4860
• mRNA Refseq: NM_000270
• Protein Refseq: NP_000261
• MIM: 164050
• UniProt ID: P00491