| Description : |
Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively). |
| Source : |
Yeast |
| Species : |
Human |
| Tag : |
His |
| Form : |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Molecular Mass : |
50.4kDa |
| Protein length : |
33-473aa |
| AA Sequence : |
GNLTVAVVLPLANTSYPWSWARVGP AVELALAQVKARPDLLPGWTVRTVL GSSENALGVCSDTAAPLAAVDLKWE HNPAVFLGPGCVYAAAPVGRFTAHW RVPLLTAGAPALGFGVKDEYALTTR AGPSYAKLGDFVAALHRRLGWERQA LMLYAYRPGDEEHCFFLVEGLFMRV RDRLNITVDHLEFAEDDLSHYTRLL RTMPRKGRVIYICSSPDAFRTLMLL ALEAGLCGEDYVFFHLDIFGQSLQG GQGPAPRRPWERGDGQDVSARQAFQ AAKIITYKDPDNPEYLEFLKQLKHL AYEQFNFTMEDGLVNTIPASFHDGL LLYIQAVTETLAHGGTVTDGENITQ RMWNRSFQGVTGYLKIDSSGDRETD FSLWDMDPENGAFRVVLNYNGTSQE LVAVSGRKLNWPLGYPPPDIPKCGF DNEDPACNQDHLSTLE |
| Purity : |
Greater than 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Storage : |
Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
