Recombinant Human NR1I2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NR1I2-3925H
• Product Overview: NR1I2 MS Standard C13 and N15-labeled recombinant protein (NP_003880) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene. Additional transcript variants exist, however, they have not been fully characterized.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.6 kDa
• AA Sequence: Tags(s)AIAXEVRPKESWNHADFVHCEDTES VPGKPSVNADEEVGGPQICRVCGDK ATGYHFNVMTCEGCKGFFRRAMKRN ARLRCPFRKGACEITRKTRRQCQAC RLRKCLESGMKKEMIMSDEAVEERR ALIKRKKSERTGTQPLGVQGLTEEQ RMMIRELMDAQMKTFDTTFSHFKNF RPGVLSSGCELPESLQAPSREEAAK WSQVRKDLCSLKVSLQLRGEDGSVW NYKPPADSGGKEIFSLLPHMADMST YMFKGIISFAKVISYFRDLPIEDQI SLLKGAAFELCQLRFNTVFNAETGT WECGRLSYCLEDTAGGFQQLLLEPM LKFHYMLKKLQLHEEEYVLMQAISL FSPDRPGVLQHRVVDQLQEQFAITL KSYIECNRPQPAHRFLFLKIMAMLT ELRSINAQHTQRLLRIQDIHPFATP LMQELFGITGSTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NR1I2 nuclear receptor subfamily 1 group I member 2 [ Homo sapiens (human) ]
• Official Symbol: NR1I2
• Synonyms: NR1I2; nuclear receptor subfamily 1, group I, member 2; nuclear receptor subfamily 1 group I member 2; BXR; ONR1; PAR2; PXR; SXR; pregnane X receptor; orphan nuclear receptor PXR; orphan nuclear receptor PAR1; steroid and xenobiotic receptor; pregnane X nuclear receptor variant 2; PAR; PRR; SAR; PAR1; PARq
• Gene ID: 8856
• mRNA Refseq: NM_003889
• Protein Refseq: NP_003880
• MIM: 603065
• UniProt ID: O75469