Recombinant Human NR3C2 overexpression cell lysate

• Cat.No.: NR3C2-17HCL
• Product Overview: Human NR3C2, transcript variant 1, was derived from Human HEK293T cell line.

Specification

• Description: This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: C-terminal Myc/DDK
• Form: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer(25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer.
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT).
• Molecular Mass: 106.9 kDa
• Storage: Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: NR3C2 nuclear receptor subfamily 3, group C, member 2 [ Homo sapiens ]
• Official Symbol: NR3C2
• Synonyms: NR3C2; nuclear receptor subfamily 3, group C, member 2; MLR; mineralocorticoid receptor; MR; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta; MCR; NR3C2VIT; FLJ41052; MGC133092
• Gene ID: 4306
• mRNA Refseq: NM_000901
• Protein Refseq: NP_000892
• MIM: 600983
• UniProt ID: P08235