Recombinant Human NUB1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NUB1-5228H
• Product Overview: NUB1 MS Standard C13 and N15-labeled recombinant protein (NP_057202) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 68.9 kDa
• AA Sequence: MAQKKYLQAKLTQFLREDRIQLWKP PYTDENKKVGLALKDLAKQYSDRLE CCENEVEKVIEEIRCKAIERGTGND NYRTTGIATIEVFLPPRLKKDRKNL LETRLHITGRELRSKIAETFGLQEN YIKIVINKKQLQLGKTLEEQGVAHN VKAMVLELKQSEEDARKNFQLEEEE QNEAKLKEKQIQRTKRGLEILAKRA AETVVDPEMTPYLDIANQTGRSIRI PPSERKALMLAMGYHEKGRAFLKRK EYGIALPCLLDADKYFCECCRELLD TVDNYAVLQLDIVWCYFRLEQLECL DDAEKKLNLAQKCFKNCYGENHQRL VHIKGNCGKEKVLFLRLYLLQGIRN YHSGNDVEAYEYLNKARQLFKELYI DPSKVDNLLQLGFTAQEARLGLRAC DGNVDHAATHITNRREELAQIRKEE KEKKRRRLENIRFLKGMGYSTHAAQ QILLSNPQMWWLNDSNPETDNRQES PSQENIDRLVYMGFDALVAEAALRV FRGNVQLAAQTLAHNGGSLPPELPL SPEDSLSPPATSPSDSAGTSSASTD EDMETEAVNEILEDIPEHEEDYLDS TLEDEEIIIAEYLSYVENRKSATKK NTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NUB1 negative regulator of ubiquitin-like proteins 1 [ Homo sapiens (human) ]
• Official Symbol: NUB1
• Synonyms: NUB1; negative regulator of ubiquitin-like proteins 1; NEDD8 ultimate buster 1; BS4; NYREN18; NEDD8 ultimate buster-1; renal carcinoma antigen NY-REN-18; NUB1L
• Gene ID: 51667
• mRNA Refseq: NM_016118
• Protein Refseq: NP_057202
• MIM: 607981
• UniProt ID: Q9Y5A7

Reviews

03/17/2023

In addition to its outstanding quality, the NUB1 Protein exhibits exceptional stability during storage and usage.

11/15/2021

With its remarkable purity and reliability, coupled with the manufacturer's excellent technical support, this protein is a crucial asset in addressing my scientific inquiries and advancing my research goals.

09/10/2019

Its ability to maintain structural integrity and stability ensures accurate imaging, enabling me to gain deeper insights into the three-dimensional organization and interactions of the proteins under investigation.

Q&As

How is NUB1 protein being studied in the context of drug development?

Scientists are exploring the potential of NUB1 as a target for novel drug development, with the aim of developing therapies for cancer and neurodegenerative diseases.

Can NUB1 protein be used as a biomarker for cancer?

Research suggests that NUB1 protein levels may serve as a potential biomarker for certain types of cancer, aiding in diagnosis and prognosis.

What neurodegenerative diseases are associated with NUB1 protein?

NUB1 has been linked to diseases such as Alzheimer's and Parkinson's, making it a potential target for therapeutic interventions.

How is NUB1 protein being targeted for cancer therapy?

Researchers are exploring ways to modulate the activity of NUB1 protein to inhibit cancer cell growth and promote tumor suppression.

How does NUB1 protein affect protein degradation pathways?

NUB1 protein has been shown to interact with the ubiquitin-proteasome system, influencing the degradation of various proteins involved in disease pathways.