Recombinant Human NUCB2, His-tagged

• Cat.No.: NUCB2-3689H
• Product Overview: Total 92 AA. MW: 10.79 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Nucleobindin-2 is a protein that in humans is encoded by the NUCB2 gene.Nucleobindin-2 is a calcium-binding EF-hand protein.
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, Ph 7.5.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: NUCB2 nucleobindin 2 [ Homo sapiens ]
• Official Symbol: NUCB2
• Synonyms: NUCB2; nucleobindin 2; NEFA; nucleobindin-2; nucleobinding 2; OTTHUMP00000231791; OTTHUMP00000231793; OTTHUMP00000231794; DNA-binding protein NEFA; gastric cancer antigen Zg4
• Gene ID: 4925
• mRNA Refseq: NM_005013
• Protein Refseq: NP_005004
• MIM: 608020
• UniProt ID: P80303
• Chromosome Location: 11p15
• Function: DNA binding; calcium ion binding