Recombinant Human Nucleosome, H3K4me1 dNuc, Biotinylated

• Cat.No.: NUC-012
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2B-O60814; H3.2-Q71DI3 *; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethyl-lysine at position 4. The nucleosome is the basic subunit of chromatin. The 601 sequence, identified by Lowary and Widom, is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5' biotin-TEG group.
• Form: Nucleosome, Recombinant Human, H3K4me1 (50 µg total mass, DNA+protein, 27.3 µg protein weight) in 10mM Tris HCl, pH 7.5, 25mM NaCl, 1mM EDTA, 2mM DTT, 20% glycerol.
• Molecular Mass: 200,620 Da
• Applications: Nucleosome, Recombinant Human, H3K4me1 are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
• Conjugation: Biotin