Recombinant Human ONECUT2 HEK293T cell lysate

• Cat.No.: ONECUT2-19HCL
• Product Overview: Human ONECUT2 over-expression lysate was derived from Human HEK293T cell line.

Specification

• Description: This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation.
• Source: Human HEK293T cell line
• Species: Human
• Tag: C-terminal Myc/DDK
• Form: The cell lysate is provided in RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4).
• Molecular Mass: 54.1 kDa
• Usage: 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
• Storage: The lysate is shipped with dry ice. Upon receiving, store the sample at -20centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20centigrade. Avoid repeated freeze-thaw cycles.
  Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: ONECUT2 one cut homeobox 2 [ Homo sapiens ]
• Official Symbol: ONECUT2
• Synonyms: ONECUT2; one cut homeobox 2; one cut domain, family member 2; one cut domain family member 2; OC 2; onecut 2; HNF-6-beta; transcription factor ONECUT-2; hepatocyte nuclear factor 6-beta; ONECUT-2 homeodomain transcription factor; OC2; OC-2; MGC120377; MGC120378
• Gene ID: 9480
• mRNA Refseq: NM_004852
• Protein Refseq: NP_004843
• MIM: 604894
• UniProt ID: O95948
• Chromosome Location: 18q21.31
• Function: sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity