Recombinant Human OTU domain, ubiquitin aldehyde binding 1 Protein, Tag Free

• Cat.No.: OTUB1-077H
• Product Overview: Recombinant human Osteoactivin/GPNMB protein (Q14956) (Glu 40-Lys 271) without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 40-271aa
• Description: Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
• Tag: Non
• Molecular Mass: 27.3 kDa
• Endotoxin: Less than 1.0 EU/μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade. It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.
• Reconstitution: Reconstitute at 250 μg/mL in sterile water.

Gene Information

• Gene Name: OTUB1 OTU domain, ubiquitin aldehyde binding 1 [ Homo sapiens (human) ]
• Official Symbol: OTUB1
• Synonyms: OTUB1; OTU domain, ubiquitin aldehyde binding 1; ubiquitin thioesterase OTUB1; FLJ20113; FLJ40710; otubain-1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTB1; OTU1; HSPC263; MGC4584; MGC111158
• Gene ID: 55611
• mRNA Refseq: NM_017670
• Protein Refseq: NP_060140
• MIM: 608337
• UniProt ID: Q96FW1