Recombinant Human PADI1 Protein, His-tagged PADI1-011H

Recombinant Human PADI1 Protein, His-tagged

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Recombinant Human PADI1 Protein, His-tagged

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Cat.No. : PADI1-011H
Product Overview : Recombinant Human PADI1 protein (2-663) with a N-terminal His-tag was expressed in E. coli
Description : This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 76.4 kDa
Protein length : 2-663
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of NH 4 + per minute at 37 centigrade in 50 mM HEPES, pH 7.7, containing 10 mM calcium chloride, 5 mM DTT, and 2 mM N-Benzoyl-L-Arginine Ethyl Ester (BAEE).
Stability : ≥ 1 year
Storage : -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride, 1mM DTT, and 20% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
SDS-PAGE analysis of PADI1 :

Lane 1: MW Markers
Lane 2: PADI1 (2 µg)
Lane 3: PADI1 (4 µg)

PADI1 Enzyme titration :

Activity of PADI1 :

PADI1 activity was determined using PADI1 Inhibitor Screening Assay Kit with 2.6 µg PADI1 and 2 mM BAEE substrate.

Gene Name : PADI1 peptidyl arginine deiminase 1 [ Homo sapiens (human) ]
Official Symbol : PADI1
Synonyms : PADI1; peptidyl arginine deiminase 1; PDI; PAD1; PDI1; HPAD10; protein-arginine deiminase type-1; hPAD-colony 10; peptidyl arginine deiminase, type I; peptidylarginine deiminase I; protein-arginine deiminase type I; EC 3.5.3.15
Gene ID : 29943
mRNA Refseq : NM_013358
Protein Refseq : NP_037490
MIM : 607934
UniProt ID : Q9ULC6

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