Recombinant Human PADI3 Protein, His-tagged PADI3-002H

Recombinant Human PADI3 Protein, His-tagged

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Recombinant Human PADI3 Protein, His-tagged

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Cat.No. : PADI3-002H
Product Overview : Recombinant Human PADI3 protein (2-664) with a N-terminal His-tag was expressed in E.coli
Description : This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes.
Source : E. coli
Species : Human
Tag : His
Molecular Mass : 76.4 kDa
Protein length : 2-664
Purity : ≥85% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmol of NH 4 + per minute at 37 centigrade in 50 mM HEPES, pH 7.7, containing 10 mM calcium chloride, 5 mM DTT, and 4 mM N-α-Benzoyl-L-Arginine ethyl ester (BAEE).
Stability : ≥ 1 year
Storage : -80 centigrade
Storage Buffer : 50 mM HEPES, pH 8.0, containing 300 mM sodium chloride, 1 mM DTT, and 10% glycerol
SDS-PAGE Analysis of PADI3 :

Lane 1: MW Markers
Lane 2: PADI3 (4 µg)
Lane 3: PADI3 (2 µg)
Lane 4: PADI3 (1 µg)

Activity of PADI3 :

PADI3 activity was determined using PADI3 Inhibitor Screening Assay Kit with 0.25 µg PADI3 and 4 mM BAEE substrate.

Gene Name : PADI3 peptidyl arginine deiminase 3 [ Homo sapiens (human) ]
Official Symbol : PADI3
Synonyms : PADI3; peptidyl arginine deiminase 3; PAD3; PDI3; UHS1; protein-arginine deiminase type-3; peptidyl arginine deiminase, type III; peptidylarginine deiminase III; protein-arginine deiminase type III; EC 3.5.3.15
Gene ID : 51702
mRNA Refseq : NM_016233
Protein Refseq : NP_057317
MIM : 606755
UniProt ID : Q9ULW8

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