Recombinant Human PAFAH1B1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PAFAH1B1-3711H
• Product Overview: PAFAH1B1 MS Standard C13 and N15-labeled recombinant protein (NP_000421) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.5 kDa
• AA Sequence: MVLSQRQRDELNRAIADYLRSNGYE EAYSVFKKEAELDVNEELDKKYAGL LEKKWTSVIRLQKKVMELESKLNEA KEEFTSGGPLGQKRDPKEWIPRPPE KYALSGHRSPVTRVIFHPVFSVMVS ASEDATIKVWDYETGDFERTLKGHT DSVQDISFDHSGKLLASCSADMTIK LWDFQGFECIRTMHGHDHNVSSVAI MPNGDHIVSASRDKTIKMWEVQTGY CVKTFTGHREWVRMVRPNQDGTLIA SCSNDQTVRVWVVATKECKAELREH EHVVECISWAPESSYSSISEATGSE TKKSGKPGPFLLSGSRDKTIKMWDV STGMCLMTLVGHDNWVRGVLFHSGG KFILSCADDKTLRVWDYKNKRCMKT LNAHEHFVTSLDFHKTAPYVVTGSV DQTVKVWECRSGPTRTRRLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PAFAH1B1 platelet activating factor acetylhydrolase 1b regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PAFAH1B1
• Synonyms: PAFAH1B1; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); MDCR, MDS, Miller Dieker syndrome chromosome region, platelet activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD), platelet activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa, platelet activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa); platelet-activating factor acetylhydrolase IB subunit alpha; LIS1; PAFAH; LIS-1; PAFAH alpha; PAF-AH alpha; PAF-AH 45 kDa subunit; lissencephaly 1 protein; lissencephaly-1 protein; PAF acetylhydrolase 45 kDa subunit; platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa); platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD); MDS; LIS2; MDCR
• Gene ID: 5048
• mRNA Refseq: NM_000430
• Protein Refseq: NP_000421
• MIM: 601545
• UniProt ID: P43034